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Monitoring mRNA production using CIMac Oligo dT analytics

mRNA has been at the forefront of both scientific and general public interests from the start of the COVID-19 pandemic. The demand for the mRNA product has been incredible for the last couple of years. However, there are still limited options available for a rapid mRNA quantification and characterization. In this work, mRNA analytics using a CIMac Oligo dT column is presented. mRNA is a specialized group of RNAs that carries the blueprints for building proteins from the cell’s DNA in the nucleus to the ribosomes in the cytoplasm. One of the features of mRNA molecules is a polyadenylated (poly(A)) tail on the 3’ end, that can be up to 250 nucleotides long. This feature enables mRNA to bind to the Oligo dT column. HPLC Oligo dT analytics provide a solution for fast and reproducible quantification of mRNA throughout all the process steps of mRNA production and purification. The presented method was validated using mFix4, an uncapped mRNA analog produced in-house, 3969 nt long molecule with a poly(A )tail length of 95 nucleotides.