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Using PATfix analytical HPLC platform to optimize lysis conditions in pDNA downstream processing

Optimizing processing steps in sc pDNA isolation is critical for obtaining good process yields as well as high product purity. HPLC with convective chromatography media (e.g. monolith) offers a rapid analytical method to characterize complex biomolecular mixtures and gives immediate feedback during process development. E coli lysis represents such a challenging step, where multiple critical quality attributes need to be identified and critical processing parameters optimized. This approach leads to better yields and product purity, allowing for simplified downstream steps. A new PATfix analytical HPLC platform presented here uses CIMac pDNA column, to separate and characterize plasmid from impurities, allowing for easy optimization of key parameters such as RNA removal.

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