Process Monitoring using Immunofluorescence and Multi Angle Light Scattering – tracking extracellular vesicles on analytical HPLC
This poster shows how Multi-Angle Light Scattering detector and Fluorescence detector couppled to PATfix analytical HPLC system can be used to track extracellular vesicles through purification process. Samples were analyzed by analytical size exclusion chromatography (SEC). On SEC cell culture components diffuze into pores of chromatographic media and are separated (mostly) based on size. Particles larger than the media pore size are excluded in the void peak. This peak represents extracellular vesicles including apoptosomes, microvesicles and exosomes as well as cell debris and aggregates.