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CIM® chromatographic monoliths enable high 1) productivity of pDNA downstream process (DSP) due to high dynamic binding capacity for pDNA in small elution volumes and short chromatographic runs; 2) high resolution power due to convective-based mass transfer.

Sample displacement mode utilizes different relative binding affinities of components in a sample mixture and separates pDNA isoforms under overloading conditions - where sc pDNA isoform acts as a displacer of oc or linear pDNA isoform.

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The CIMacTM antibody immobilization platform enables an elegant immobilization of antibodies, which can be used as immunosorbents in specific diagnostic applications as well as in downstream processes. In this work we show the dependence of the coupling strategy on CIM monolith with the chromatographic efficiency of final immunoaffinity adsorbent. Different activation chemistries  were tested for the immobilization of two model monoclonal antibodies (mAbs) with subsequent chromatographic characterization of the affinity support.

Column used for this application note were CIMac CDI, AE, EDA, HDZ, rpA.

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During recombinant adeno associated virus (rAAV) downstream processing, a large amount of host-cell and product related impurities needs to be removed from the product. Succesful process on laboratory scale, such as Cesium chloride purification, lacks scalability when the process is due to be transfered to larger industrial scale. The aim of the study was to develop robust, fast and effective rAAV virus purification platform, which can be used for several AAV serotypes with various inserts. Lysed harvest and supernatant of rAAV9 were first captured and concentrated on CIMmultus™ OH column, followed by intermediate step on CIMmultus™ SO3 column and further polishing on CIMmultus™ QA column. Derived purity of industrial scale monolith purification product was compared to laboratory scale purification.

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H-Bond ADC is the first of a new class of chromatography ligands from BIA separations that exploits hydrogen bonding as the dominant mode of biomolecule retention. The ligand consists of a terminal series of hydrogen donors grafted to a root series of hydrogen acceptors.

H-Bond ADC brings a unique new selectivity to all fractionation tasks but is especially distinctive in its ability to retain large biomolecules more strongly than small ones. This can be of great value for removing fragments, aggregates, and viruses from protein products, or for removing proteins and other small contaminants from large biologics like viruses and extra-cellular vesicles.

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F.A.Q.

Yes. Make sure that either the inlet or the outlet of the column is open to prevent excess pressure buildup. Autoclaving monolithic columns for aseptic use is recommended. Prior to disposal, monoliths can also be autoclaved. For specifics, please consult help@biaseparations.com.

Yes.