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2010

Bacteriophages, viruses that infect bacteria, are being used as antibacterial agents, in phage display screening, as gene therapy delivery systems, and for bacteria typing. To use phages in these applications, they must be free of all impurities. A purification and concentration process was recently developed using an ion exchange monolithic column [1]. One of the key challenges faced in phage purification is the monitoring of genomic DNA (gDNA) released to the growth medium which can interfere with the various applications of phages. CIMac™ DEAE Analytical Columns can be used to monitor the fermentation process, evaluate the amount of degraded gDNA to determine the optimal fermentation endpoint and then to efficiently purify the phage particles.

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The demand for monoclonal antibodies is invariably increasing on an annual basis. To satisfy increasing demands, faster and cheaper ways of manufacturing are explored. A quest for alternative paths in manufacturing not only requires development of most economical manufacturing process, but also rapid method development and development of good analytics for monitoring of manufacturing. For a quickly developed process, the use of reliable and fast analytical techniques are crucial. Moreover, this analytical technique should than be preferably used also for in-process control during manufacturing stage.


Here we present fast and reliable method for processing and analyzing IgG, IgA ang IgM using CIM® QA Disk Monolithic Column, which thrive upon speed, repeatability and high capacity.

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2008

A Hemoglobin A1c reference standard was loaded on CIM® SO3 monolithic column and eluted in a mixed stepwise and linear gradient. HbA1a, HbA1b and HbA0 variants were separated and a complete determination of HbA1c (including equilibration) was obtained within 1.1 minute.

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A mixture of IgG, HSA and IgM standard was loaded on CIM® EDA Disk and eluted in linear salt gradient at a flow rate of 4 mL/min (12 CV/min). A complete separation of IgM from IgG and HSA was obtained within 1.5 minute.

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A mixture of 8mer, 10mer, 12mer, 14mer, 15mer and 16mer Oligodeoxynucleotides was loaded on CIM® DEAE Disk and eluted in linear gradient mode at a flow rate of 6 mL/min (17 CV/min). Separation of all nucleotides could be accomplished within 60 seconds.

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Immunoaffinity columns were prepared by immobilization of Protein G on CIM® Epoxy Disk, CIM® Epoxy tube (1 mL) and an activated, particle based agarose support. A comparison of productivity was performed by loading centrifuged human plasma and resulted in superior productivity of CIM® monolithic supports.

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1999

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