A fast and reproducible HPLC method for quantification of Adenoviruses
Determining the concentration of viruses is a crucial step in any production process. The most commonly used methods for virus quantification are either based on the infectivity of the virus (plaque assay, TCID50) determination of their genomic material (qPCR), or protein content (SRID, ELISA) and are very cumbersome and time consuming. HPLC analytical methods represent a fast alternative to these assays since they provide information on the virus content and purity in a matter of minutes. In addition to that, the data obtained is very reproducible and accurate.
For any kind of quantification, a calibration curve obtained with a virus standard is needed. The work presented in this application note shows the excellent performance of the CIMac™ Adeno Analytical Column – a monolith based anion exchange column, designed for fast and reproducible analyses of adenoviruses.