Speciation of Pt in serum of cancer patients on CLC monolithic columns with ICP-MS detection
In speciation analysis of anticancer metallodrugs, size exclusion chromatography (SEC) coupled to inductively coupled plasma mass spectrometer (ICP-MS) is frequently used. The size exclusion coupled ICP-MS is troubled by low resolution, long time of analysis (up to 60 min) and tendency of some metals to irreversibly adsorb on the surface of the stationary phase. Higher resolution can be achieved by ion-exchange particle columns, but this doesn’t address time frame of the analysis and brings in additional bottlenecks in the form of the potential clogging issues connected to the particle pore diameter.
Alternatively, monolithic ion-exchangers can replace the particle-based columns. Monoliths enable improved protein separation facilitated by increased mass permeability, allowing for higher flow rates while preserving separation efficiency at low backpressures. Subsequently, time of the chromatographic separation can be shortened2 – making kinetic studies less troublesome. In addition, monolithic columns are more robust, can undergo rigorous cleaning procedures and enable analyses of large series of clinical samples.