Versatility of CIM® monoliths for covalent immobilization of antibodies

The CIMacTM antibody immobilization platform enables an elegant immobilization of antibodies, which can be used as immunosorbents in specific diagnostic applications as well as in downstream processes. In this work we show the dependence of the coupling strategy on CIM monolith with the chromatographic efficiency of final immunoaffinity adsorbent. Different activation chemistries  were tested for the immobilization of two model monoclonal antibodies (mAbs) with subsequent chromatographic characterization of the affinity support.

Column used for this application note were CIMac CDI, AE, EDA, HDZ, rpA.