CIMac™ Analytical Columns are short bed, high performance monolithic columns offering all the advantages of CIM® monolithic technology. They are primarily intended for fast, efficient, and reproducible separations of biomolecules like large proteins, plasmid DNA, and viral particles. Their small volume and short column length allow the operation at high volumetric flow rates (from 1 to 30 column volumes/min), enabling to receive the information about the product quantity and purity in just a few minutes.
Figure: Separation on CIMac™ SO3 - 6 proteins at three different flow rates
These columns are pre-packed in dedicated stainless steel housings and allow user-friendly connections to HPLC equipment. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
Features and Benefits:
- Low volume monolithic stationary phase in a stainless steel housing with low dead volume and excellent flow distribution.
High efficiency and high sensitivity of the column. Very shallow linear gradients can be applied without deteriorating the column performance.
- Small bed volume with high binding capacity.
Extremely fast separations speed up method development and analysis time.
- Flow unaffected resolution.
Enables changing the flow rate without any deterioration of the separation. In-process control of various process streams and final control of pure samples can be done within only a few minutes. Enables PAT technology.
- High chemical stability.
Enables regeneration and cleaning under extreme conditions such as 1 M strong acids and bases, organic solvents and highly concentrated salt solutions.
- High sensitivity.
Sensitivity of CIMac™ Analytical columns is additionally improved due to a special design of the CIMac™ columns.
Low sample and buffer consumption.