SCALE-UP CALCULATOR >

CIM® Method Transfer

a quick and easy way to Scale up or down your chromatographic method

Method Transfer Calculator - Use Now

A major benefit of using CIM® monolithic columns is the ability to quickly and easily Scale Up or Scale Down your optimized method. This is possible because the monolith’s structure, especially the pore size distribution, is the same regardless of the column’s size. This ensures that each column has the same resolution and an identical performance and purification profile no matter if it used in discovery, production, or QA. Since CIM® monolithic supports are optimized for the separation of large molecules, gradient chromatographic methods are normally applied. With this in mind, Milavec et al derived a very simple equation for scaling up/down an optimized gradient.


CIM® Column
(Disk or Tube)
Gradient Time
Column
Void Volume
Flow Rate
Column Length
Column1
(Starting)
tg1
V1
F1
L1
Column2
(Target)
tg2
V2
F2
L2

The elegance of this equation is that the only variable parameter is the gradient time (tg). The flow rate (F) becomes variable only when a rate less then the maximum (shown in the table below) is utilized. It is important to note that some fine tuning of the gradient may be required after the method is transferred.

Monolithic Support Parameters used for Scale Up/Down Calculation

CIM®
Disk
Monolith Volume (ml)
Void Volume
V (ml)
Max Flow Rate
F (ml/min)
Column Length
L (cm)
1
0.34
0.21
10
0.3
2
0.68
0.42
10
0.6
3
1.02
0.63
10
0.9
4
1.36
0.84
10
1.2

CIM®
Tube
Monolith Volume (ml)
Void Volume
V (ml)
Max Flow Rate
F (ml/min)
Column Length
L (cm)
1

1

1

3
0.42
8
8
6
40
0.65
80
80
78
250
1.6
800
800
670
2000
2.1
8000
8000
- 
10000
2.2

An example of a standard protein mixture being scaled up from a CIM® DEAE research column ( 1 Disk) to an industrial preparative column (800ml) can be seen in the following Chromatograms (JPG, 53kB).