PRODUCTS > CIM® Analytical Columns

CIMac pDNA Analytical Column

Cat. No.: 150.0001

CIMac™ pDNA Analytical Columns were specifically developed in order to monitor a plasmid DNA quantity and purity throughout the downstream processing of plasmid DNA. Besides high performance their inherent features enable highly reproducible separations of different plasmid DNA conformations at extremely high speeds. Their small volume and short column length allow the operation at high volumetric flow rates (up to 10 column volumes/min) thus enabling receiving the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ pDNA Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.

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Features and Benefits:

  • Low volume monolithic stationary phase in a stainless steel housing with a low dead volume and excellent flow distribution.
High efficiency and high sensitivity of the column. Very shallow linear gradients can be applied without deteriorating the column performance.
  • Small bed volume with high binding capacity
Extremely fast separations speed up method development and anaylsis time.
  • Flow unaffected resolution
 Enables changing the flow rate without any deterioration of the separation. In-process control of various process streams and final control of the pure samples can be done within only a few minutes. Enabling PAT technology.
  • High chemical stability.
Enables regeneration and cleaning under extreme conditions, such as 1.0 M strong acids and bases, organic solvents and highly concentrated salt solutions.
  • High sensitivity
It keeps the benefits of the CIM® Disk Monolithic Columns, but the sensitivity is additionally improved due to a special design of the CIMacTM columns.
The CIMacTM Analytical Columns can be used for both process screening and optimization as well as for the HPLC process analytics (in-process and final control).
Low sample and buffer consumption.
  • Optimized in terms of column geometry, structure and ligand density.
Highly efficient separations of different pDNA conformations.
  • Excellent lifetime and repeatability of pDNA separations.
More than 1000 analytical injections of a purified plasmid DNA sample and more than 200 analytical injections of an alkaline lysate containing plasmid DNA were performed without any deterioration in column performance.
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Dimensions and Operating Conditions:

Monolith dimensions

  • diameter: 5.2 mm
  • legth: 15.0 mm
  • bed volume: 0.32 mL
  • ring colour: green and yellow

Operating conditions

  • flow rates: recommended: 0.2 to 2 mL/min (1 to 10 cm/min); Maximum: 3 mL/min (15 cm/min)
  • pressure: 100 bar (10 MPa)
  • temperature: 4 °C (39 °F) to 40 °C (104 °F)
  • recommended pH:
    working range 3–9
    cleaning-in-place 1–14

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