PRODUCTS > CIM® Industrial Columns

CIM® EDA-8f mL Ion Exchange Chromatography Column

Cat. No.: 430.5116

General Description:

CIM® EDA immobilization columns are amine activated monolithic supports obtained by reacting the native epoxy groups with ethylene diamine. They are used for fast, highly efficient separations of large molecules such as proteins and DNA, as well as smaller molecules such as peptides.

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Features and Benefits:

  • Flow unaffected resolution
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and inustrial scale.
Buffer A: 20 mM Tris-HCl, pH 7.4
Buffer B: buffer A + 1 M NaCl, pH 7.4
Gradient: 1100 % buffer A for 30 s linear gradient from 0 – 80 % buffer B in 3 minutes
Flow rate: 16 mL/min
Detection: UV at 280 nm 		Sample: test protein solution (0.5 mg Mb, 1.5 mg CA, 2 mg STI in 1 mL of buffer A)
Inj. Volume: 100 µL
Peaks:
1. Myoglobin (Mb)
2. Conalbumin (CA)
3. Soy. Trypsin Inhibitor (STI)

Dimensions and Operating Conditions:

Monolith Dimensions  
  • Outer diameter, Do: 15.0 mm
  • Inner diameter, Di: 6.5 mm
  • Length, L: 56.0 mm
  • Bed volume, V: 8.0 mL
Operating Conditions 
  • Fow rates: up to 400 mL/min (uav = 1340 cm/h)
    WARNING: Reversing the flow direction can seriously damage the column!
  • Pressure: up to 20 bar (2 Mpa)
  • Temperature: 4 °C (39 °F) to 50 °C (122 °F)
  • Recommended Ph:
    working range 2–13*
    cleaning-in-place 1–14* (e. g. 1 M NaOH)
* Valid for the CIM® EDA Disk.

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