PRODUCTS > CIM® Industrial Columns

CIM® CM-8f mL Tube Monolithic Column

Cat. No.:  431.6170

General Description:

The CIM® CM-8f high throughput Tube Monolithic Column consists of a unique polymeric monolith encased in a specially designed housing. This column is used for extremely fast, highly efficient separations of large molecules such as proteins, viruses, as well as smaller molecules such as peptides. The design of the column allows the mobile phase to run in a radial direction from the outer to the inner surface. In this way, resolution is preserved during scale-up while maintaining the short separation layer length. The short monolithic length and the specially engineered highly porous structure allow operations at elevated flow rates with low-pressure drops (only few bars).

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Features and Benefits:

  • Flow unaffected resolution
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and industrial scale.
Buffer A: 20 mM Tris-HCl, pH 7.4
Buffer B: buffer A + 1 M NaCl, pH 7.4
Gradient: 100 % buffer A for 30 s
Peaks: linear gradient from 0 – 50 % (Cyt.)
buffer B in 3 minutes
Flow rate: 16 mL/min
Detection: UV at 280 nm 		Sample: test protein solution
(0.7 mg Mb, 0.7 mg Cyt., 0.7 mg Lys in 1 mL of buffer A)
Inj. Volume: 100 µL
Peaks:
1. Myoglobin (Mb) 
Cytochrome C (Cyt.)
3. Lysozyme (Lys.)

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