PRODUCTS > CIM® Industrial Columns

CIM® SO3-8f Tube Monolithic Column

Cat. No.:  430.5113

General Description:

CIM® SO3-8f Tube Monolithic Column is a strong cation exchanger used for extremely fast and highly efficient separations of large molecules such as proteins, as well as smaller molecules such as peptides. It is also suitable for virus purification.

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Features and Benefits:

  • Flow unaffected resolution
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and industrial scale.
 

Buffer A:
20 mM Tris-HCl, pH 8.0
Buffer B:
Buffer A + 2 M NaCl, pH 8.0
Gradient:
100 % Buffer A for 30 s
Linear gradient from 0 – 60 %
Buffer B in 3 minutes
Flow rate: 16 mL/min
Detection: UV at 280 nm

Sample:
Test protein solution (0.7 mg Mb, 0.7 mg Cyt., 0.7 mg Lys in 1 mL of buffer A)
Inj. Volume:
100 µL
Peaks:
1. Myoglobin (Mb)
2. Cytochrome C (Cyt.)
3. Lysozyme (Lys.)

Dimensions and Operating Conditions:

Monolith dimensions

  • Outer diameter, Do: 15.0 mm
  • Inner diameter, Di: 6.5 mm
  • Length, L: 56.0 mm
  • Bed volume, V: 8.0 mL

Operating conditions

  • Flow rates: up to 400 mL/min (uav = 1340 cm/h)
    WARNING: Reversing the flow direction can seriously damage the column!
  • Pressure: up to 20 bar (2 Mpa)
  • Temperature: 4 °C (39 °F) to 50 °C (122 °F)
  • Recommended Ph:
    working range 2–13
    cleaning-in-place 1–14 (e. g. 1 M NaOH)
    WARNING:     Avoid prolonged use at elevated temperatures!

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