PRODUCTS > CIM® Industrial Columns

CIM® DEAE 8-f Tube Monolithic Column

Cat. No.:  430.5114

General Description:

CIM® DEAE 8f Tube Monolithic Column is a weak anion exchanger. This column is used for extremely fast, highly efficient separations of large molecules such as proteins or DNA, as well as smaller molecules such as peptides . Applications vary from the field of human plasma/serum fractionation industry to pDNA purification. It has a high pDNA binding capacity (13 mg/mL) and it’s highly selective. Drug Master File for CIM® DEAE Monolithic Column is available.

Learn more on CIM® Tube technology >>

Features and Benefits:

  • Flow unaffected resolution
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and industrial scale.

Buffer A:
20 mM Tris-HCl, pH 7.4
Buffer B:
 Buffer A + 1 M NaCl
Gradient:
100 % Buffer A for 30 s
Linear gradient from 0 – 70 %
Buffer B in 3 minutes
Flow rate: 16 mL/min
Detection: UV at 280 nm

Sample:
Test protein solution (0.5 mg Mb, 1.5 mg CA, 2 mg STI in 1 mL of buffer A)
Inj. Volume:
100 µL
Peaks:
1. Myoglobin (Mb)
2. Conalbumin (CA)
3. Soy. Trypsin Inhibitor (STI)

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