PRODUCTS > CIM® Laboratory Columns

CIM® DEAE Disk Monolithic Column

Cat. No.:  210.5114

General Description:

CIM® DEAE Monolithic Column is a weak anion exchanger. It is suitable for a wide range of applications varying from the field of human plasma/serum fractionation industry to pDNA purification. It has a high pDNA binding capacity (13 mg/mL) and it’s highly selective. Drug Master File for CIM® DEAE Monolithic Column is available.

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Features and Benefits:

  • Flow unaffected resolution
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and industrial scale.
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Buffer A: 20 mM Tris-HCl, pH 7.4
Buffer B: buffer A + 1 M NaCl, pH 7.4
Gradient: 100 % buffer A for 6 s, then  from 0 - 70 % buffer B in 30 s.
Flow rate: 4 ml/min
Detection: UV at 280 nm 		Sample: test protein solution (0.5 mg Mb, 1.5 mg CA and 2 mg STI in 1 ml of buffer A)
Inj. Volume: 20 µl
Peaks:
1. Myoglobin (Mb) 
2. Conalbumin (CA)
3. Soy. Trypsin Inhibitor (STI)
4. Solvent

Dimensions and Operating Conditions:

Monolith dimensions
  • diameter: 12 mm
  • thickness: 3 mm
  • disk volume: 0.34ml
  • ring colour: green
Operating conditions
  • flow rates: up to 10 ml/min (530cm/h)
  • pressure: up to 50 bar (5 MPa)
  • temperature: 4°C (39°F) to 50°C (122°F)
  • Recommended Ph:
    working range 3–9
    cleaning-in-place 2–14 (e. g. 1MNaOH)

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