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PRODUCTS > CIM^® Laboratory Columns

CIM^® C4 A Disk Monolithic Column

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About the CIM^® C4 A Disks

The CIM^® C4 A (very low ligand density) Butyl Disk consists of a unique monolithic stationary phase that is easily placed in a dedicated plastic housing forming a CIM^® Disk Monolithic Column. These columns are used for fast, highly efficient separations of large molecules such as proteins or DNA, as well as smaller molecules such as peptides. The short monolithic length and the specially engineered highly porous structure allow operations at elevated flow rates with low-pressure drops (only a few bars).
The following information is being provided to ensure proper product care and maximal product life. All other information can be found in the Instruction Manual.

Color code:	yellow
Catalog number:	210.8134
Disk chemistry:	butyl
Ligand density:	very low
Support matrix:	poly (glycidyl methacrylate-co-ethylene dimethacrylate-co-butyl methacrylate)
Disk dimensions:	diameter: 13.4 mm; thickness: 2.5 mm; bed volume: 0.35 mL
Fitting ring:	high density polyethylene (PE-HD); inner diameter: 13.4 mm, outer diameter: 16 mm
Working flow rates:	up to 10 mL/min (530 cm/h)
Working system pressure:	up to 50 bar (5 MPa); WARNING: Do not exceed the maximum allowed pressure as this might seriously damage your column!
Temperature stability:	4 °C (39 °F) to 50 °C (122 °F) WARNING: Avoid prolonged use at elevated temperatures!
Recommended pH:	working range      1–12 cleaning-in-place 1–14 (e. g. 1 M NaOH)

Caring for the CIM^® C4 A Disk Monolithic Columns

Cleaning In Place (CIP)

The build up of contaminants on the monolithic column may cause loss of resolution and binding capacity, increased back pressure, or a complete blockage of the column. A specific CIP protocol should be designed according to the type of contaminants that are present in your sample. However, in most cases, the following procedures can be used:

1. Removal of precipitated protein

• Wash with 10 column volumes of 1.0 M NaOH.
  Note: Reverse the flow direction and use low enough flow rates to expose the column to the cleaning reagent for several minutes.
• Wash with 10 column volumes of deionized water at the working flow rate.
• Wash with 10 column volumes of 20 % ethanol at the working flow rate.
• Re-equilibrate with 10 column volumes of the working mobile phase (buffer) at the working flow rate.

2. Removal of strongly bound hydrophobic proteins or lipids
Reverse the flow direction and:

• Wash with 10 column volumes of deionized water at one-half of the working flow rate.
• Wash with 10 column volumes of 2-propanol (30 % v/v) or ethanol (70 % v/v) at one-half of the working flow rate.
• Wash with 5 column volumes of 20 % ethanol at the working flow rate.
• Re-equilibrate with 10 column volumes of the working mobile phase (buffer) at the working flow rate.

Sanitization

Sanitization of the monolithic column is obtained by thoroughly washing it with 1.0 M NaOH for at least 1 hour at room temperature.

Storage of the CIM^® C4 A Disk Monolithic Columns

1. Short term storage
CIM^® C4 A Disk Monolithic Columns should be stored at a temperature of +4 °C to +8 °C in the presence of a suitable bacteriostatic agent, e. g. 20 % ethanol.

2. Long term storage
CIM^® C4 A Disk should be thoroughly cleaned (CIP) at the end of the working day and removed from the housing. The disk should be stored in a jar with 20 % ethanol.