PRODUCTS > CIM® Laboratory Columns

CIM® C4 A Disk Monolithic Column


Cat. No.: 210.8134

General Description:

The CIM® C4 A is a butyl disk with very low ligand density. This disk is used for fast, highly efficient separations of large molecules such as proteins or DNA, as well as smaller molecules such as peptides. 

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Features and Benefits:

  • Flow unaffected resolution.
Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity.
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop.
High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps)
  • Same material and design accross all products.
Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep to industrial scale.
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Buffer A: 0.05 M phosphate buffer
+ 1 M ammonium sulfate, pH 7.0
Buffer B: deionized water
Gradient: 10 s isocratic at 100 %, 18 s at 60 % and 14 s at 0 % buffer A.
Flow rate: 4 ml/min
Detection: UV at 280 nm 		Sample: test protein solution (2 mg Mb, 3 mg CA and 5 mg STI in 1 ml of buffer A)
Inj. Volume: 20 µl
Peaks:
1. Myoglobin (Mb) and 14 s at 0 % buffer A.
2. Conalbumin (CA)
3. Soy. Trypsin Inhibitor (STI)
4. Solvent

Dimensions and Operating Conditions:

Monolith conditions
  • inner diameter: 13.4 mm
  • thickness: 2.5 mm
  • disk volume: 0.35 mL
  • ring colour: yellow
Operating conditions
  • flow rates: up to 10 mL/min (530cm/h)
  • pressure: up to 50 bar (5 MPa)
  • temperature: 4°C (39°F) to 50°C (122°F)
  • Recommended Ph:
    working range 1–12
    cleaning-in-place 1–14 (e. g. 1 M NaOH)

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