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Hydrophobic

Hydrophobic interaction chromatography (HIC) capitalizes the interaction between non-polar molecules and a hydrophobic stationary phase. Hydrophobic patches of your molecule interact with mildly hydrophobic ligands. The retention of biomolecules is promoted by salt; therefore, the mobile phase contains high salt concentration. Desorption in return is effected by decreasing salt concentration. Due to its exceptional separation power and the maintenance of biologic activity despite hydrophobic conditions, HIC became an integrated part of chromatographic solutions. We offer a selection of three different HIC monolithic columns.

  • C4 A
  • C4 HLD
  • OH