CIM® DEAE-1 mL tube is a cylindrical-shaped monolithic column with a column volume of 1 mL. It is encased in a specially designed housing and is supplied ready to use. CIM® DEAE-1 mL is intended for initial screening, method development, and purifications at laboratory scale. It enables you to develop robust methods that are easily scalable. DEAE (diethylamine) is a weak type of anion exchange group, fully charged between pH 3-9. It selectively binds biomolecules with a predominant negative charge over a working pH range of 3-9. DEAE is a versatile chemistry and has proven to be excellent for:
- Various applications during capture step
- Purification and concentration of viruses, VLPs, and large proteins
- Any kind of pDNA purification; it has a compelling high-binding capacity for pDNA and is your chemistry of choice.
Due to the difference in chemistry of QA (strong anion exchanger) and DEAE (weak anion exchanger), selectivities for a certain molecule are different.
Read more: radial flow, upscaling