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IDA-Cu

Affinity

IDA-Cu (iminodiacetic acid-copper(II) complex) is an immobilized metal affinity ligand (IMAC) with preloaded copper(II) cations. As such it is ready to use for the purification of histidine-tagged proteins and proteins with high lysine content.

CIMmultus™ Disposable

CIMmultus™ IDA-Cu-1mL (1.3 μm)
CIMmultus™ IDA-Cu-8 mL (1.3 μm)
CIMmultus™ IDA-Cu-80 mL(1.3 μm)

CIMmultus™ is the new and improved line of monolithic columns, distinguished by 2 µm flow-through channels, designed for working with large proteins (IgG, IgM, PEGylated proteins), viruses, VLPs, phages, and pDNA. Due to their performance properties, CIMmultus™ columns can be used as single-use disposable or multi-use columns. Read more...
CIM Line

CIM® IDA-Cu - 1 mL
CIM® IDA-Cu - 8 mL
CIM® IDA-Cu - 80 mL
CIM® IDA-Cu - 0.34 mL

CIM® line monolithic columns are the first generation of monolithic products and the precursor of the optimized CIMmultus™ line of products. Distinguished by 1.5 µm flow-through channels, CIM® line is available in polypropylene and stainless steel housings and intended for liquid chromatographic purification and concentration of large biomolecules.
CIM® line is currently commercially available, but it will soon be completely replaced by its enhanced successor, the optimized CIMmultus™ line.

Affinity

Affinity chromatography is based on the biospecific interaction between a biomolecule (commonly antibody) and its native or synthetic ligand. The effective ligands are immobilized on CIM® monoliths and associate with your antibodies in a highly specific and reversible manner. Association takes place at physiologic pH, while dissociation is most commonly effected by a pH shift. The strength of interaction strongly depends on the type of ligand and antibody. Affinity chromatography has become state of the art in antibody purification. We provide a selection of affinity monolithic columns for efficient purification of IgG and IgM.
Ion-Exchange

Ion exchange chromatography (IEC) is based on electrostatic interaction between a charged molecule and an oppositely charged stationary phase. Depending on the type of charge, one discriminates between anion (AEC) and cation exchange chromatography (CEC). Your molecule binds at low salt concentration, while desorption is effected by increasing salt concentration. Ion exchange ligands are classified as either strong or weak, depending on their charge as a function of pH. IEC is a versatile and extensively used tool in downstream processing, and we contribute with a variety of weak and strong AEC and CEC monolithic columns.
Hydrophobic

Hydrophobic interaction chromatography (HIC) capitalizes the interaction between non-polar molecules and a hydrophobic stationary phase. Hydrophobic patches of your molecule interact with mildly hydrophobic ligands. The retention of biomolecules is promoted by salt; therefore, the mobile phase contains high salt concentration. Desorption in return is effected by decreasing salt concentration. Due to its exceptional separation power and the maintenance of biologic activity despite hydrophobic conditions, HIC became an integrated part of chromatographic solutions. We offer a selection of three different HIC monolithic columns.
Activated

Any individual application employing a biospecific, non-commercial ligand-protein or ligand-peptide interaction requests a tailor-made solution. By coupling your specific ligand to an activated CIM® monolithic surface via a hydrolytically stable covalent linkage, you will obtain your custom made affinity column. The choice of coupling method depends on the ligand to be immobilized; i.e., the chemistry of its active groups, its pH and temperature stability, reactivity, etc. We support your individual needs by providing different activated CIM® monolithic columns.

IDA

IDA (iminodiacetic acid) is intended for immobilized metal ion affinity chromatography (IMAC). It represents a chelator, which forms a coordination complex in combination with metal ions (Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺). Histidine-tagged proteins are selectively retained by metal ions such as nickel and cobalt. Besides its use as affinity ligand for tagged proteins, monolithic IMAC is applicable for purification of recombinant antibodies. In general, IMAC purification is your preferred technique when high yields of pure and active protein are required.
IDA-Cu

IDA-Cu (iminodiacetic acid-copper(II) complex) is an immobilized metal affinity ligand (IMAC) with preloaded copper(II) cations. As such it is ready to use for the purification of histidine-tagged proteins and proteins with high lysine content.
n-Prot A

The n-Protein A (native protein A from Staphylococcus aureus) is an affinity ligand, used for fast, highly efficient purification of the immunoglobulin G. The Fc portion of antibodies (IgG class) is bound to the n-Protein A without affecting the antigen binding. In an alternative application, the n-Protein A can be used as a platform for binding specific antibodies with their consequent crosslinking. Using this approach, you can prepare your monolithic affinity column for capturing antigens for specific antibodies.
r-Prot A

The r-Protein A (recombinant protein A produced in E. coli) is an affinity ligand, used for fast, highly efficient purification of the immunoglobulin G. The Fc portion of antibodies (IgG class) is bound to the r-Protein A without affecting the antigen binding. In an alternative application, the r-Protein A can be used as a platform for binding specific antibodies with their consequent crosslinking. Using this approach, you can prepare your monolithic affinity column for capturing antigens for specific antibodies.
r-Prot G

The r-Protein G (recombinant protein G produced in E. coli) is an affinity ligand, used for fast, highly efficient purification of the immunoglobulin G. The Fc portion of antibodies (IgG class) is bound to the r-Protein G without affecting the antigen binding. In an alternative application, the r-Protein G can be used as a platform for binding specific antibodies with their consequent crosslinking. Using this approach, you can prepare your monolithic affinity column for capturing antigens for specific antibodies. The range of polyclonal IgG's that strongly bind to the r-Protein G is wider than with n-Protein A or r-Protein A.
r-Prot L

The r-Protein L (recombinant protein L produced in E. coli) is an affinity ligand, used for fast, highly efficient purification of different immunoglobulins. In contrast to the protein A and protein G, the r-protein L binds to the kappa light chain of antibodies, and as such it can be specifically used for IgG, IgA, and IgM antibodies containing only kappa chains. Species whose antibodies contain almost exclusively lambda chains (bovine, goat, sheep, and horse) will not bind well to the Protein L.
Custom affinities

If you application requires specific column chemistries, we can prepare custom-made affinity columns upon request.

Please, contact our sales department.