- What is the matrix chemistry of most of the CIM® monolithic products?
Polyglycidylmethacrylate-co-ethyleneglycoldimethacrylate.
However, recently we also introduced a new monolithic material based on a styrene-divinylbenzene polymer for fast and efficient Reversed Phase separation of large molecules at RT.
- What are the dimensions of the monolithic columns?
Disk: diameter: 16 mm, thickness: 3 mm, monolithic volume: 0.34 mL; housing dimensions: diameter: 30 mm, length: 65 mm;
8 mL tube: active bed: outer diameter: 15 mm, inner diameter: 1.5 mm, length: 45 mm, volume: 8 mL; housing dimensions: diameter: 26 mm, length: 100 mm;
80 mL tube: active bed: outer diameter: 35 mm, inner diameter: 3.0 mm, length: 85 mm, volume: 80 mL; housing dimensions: diameter: 44 mm, length: 150 mm.
- What is the maximal operating pressure for the disk monolithic columns?
4 MPa.
- What are the advantages of CIM® monolithic columns versus conventional columns?
Extremely fast separation, flow unaffected resolution, flow unaffected dynamic binding capacity, low back pressure, user-exchangeable monolithic support, user-adjustable column length, versatility, easy to use.
- How much protein can be loaded on the monolithic columns?
- Disk: up to 10 mg
- 8 mL tube: up to 200 mg
- 80 mL tube: up to 2 g
- How can I clean or regenerate the CIM® column?
With 1 M NaOH.
- How many disks can be placed in one column to increase resolution?
Up to 4 disks.
- How large is the specific surface area inside of a CIM® monolith?
The surface area is around 40 m2/g down to the pore size of 15 nm.
- What is the pH stability of CIM® monolithic columns?
CIM® disk columns are stable in a pH range from 1-13.
- Which chemistries are available for CIM®?
Ion Exchange, Hydrophobic Interaction, Reversed Phase and Affinity.
- Can CIM® material be run on any HPLC/FPLC system?
Yes, CIM® columns can be used on any standard HPLC/FPLC systems. They contain VALCO-type adapters for connection.
- Can we run CIM® disks with laboratory pumps?
Yes, at low flow rates.
- Do we have to use an in-line filter?
Yes, an in-line filter is recommended.
- Do we have to filter the sample prior the application on a CIM® column? Which pore size?
Yes, a syringe filter of 0.45 µm pore size is recommended.
- Can we run the ion-exchange CIM® materials with the same loading/washing/eluting system as with gel based material?
Yes.
- How to store Affinity CIM® disks?
It is recommended to store them in buffer containing 0.02 % NaN3 or 20 % ethanol as preservative.
- Up to which flow rates the High-Throughput 80 mL CIM® tube monolithic column can be operated?
Up to 250 mL/min (> 3 CV/min).
- Why does the manometer on my LC system indicate a higher pressure drop with my CIM® monolithic column then with a traditional particulate column? Don't CIM® monolithic columns have lower pressure drops?
The pressure drop indicated on the system manometer represents the pressure drop of the CIM® monolithic column plus the pressure drop of the HPLC system (capillaries, valves, injector, detector,...).
The higher flow rates utilized with CIM® monolithic columns results in an increased pressure drop on the HPLC system. Thus, giving the illusion that the CIM® column is increasing the overall pressure drop. To prove this, first connect the HPLC system without the monolithic column (with e.g. a zero dead volume connector), run the mobile phase at the maximum flow rate for the CIM® monolithic column and determine the background (system) pressure drop. You will notice that it is higher then what you would normally see at a lower flow rate.
Then, repeat the procedure using the CIM® monolithic column instead of the connector. Read the pressure drop. The difference between both readings will give you the actual pressure drop on the monolithic column under specified conditions. You will see that it is the system and not the column that accounts for the increase in system pressure.
- What is the matrix chemistry of most of the CIM® monolithic products?
No.
- How do I easily and quickly transfer my method from Research to a Preparative Scale?
The following article Transfer of gradient chromatographic methods for protein separation to Convective Interaction Media monolithic columns (
pdf, 233 KB) by Milavec et al will show you how quickly and easily this can be done. If you have any questions after you read this article, please
contact us.
- Why is the backpressure increasing on my system after I apply a crude sample to your disk monolithic column?
An increase in backpressure could be due to either the binding of lipids to the monolith matrix or unspecific binding of the sample contents to the frit. To decrease the likelihood of this happening, it is important to make sure that your samples have been pre-filtered before entering the column and to ensure the removal of lipids from the sample. However, if this is observed, the first step would be to follow the Cleaning in Place (CIP) Procedure that is described in the Product Specific Information Sheet (PSIS) that came with your column. If this does not work, then it is most likely due to an unspecific interaction with the frit which should then be replaced.
