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FIELDS
OF USE
CIM®
monolithic supports can be used for a huge variety of
laboratory steps, like e.g. separation and purification
of peptides, proteins, oligonucleotides and polynucleotides;
fast-in process control during production and downstream
processing; solid phase extraction and sample enrichment;
quality control; fast method development as well as
for biosensors and for bioconversions. This record provides
an overview of most common fields of use for CIM®
monolithic supports grouped by various categories.
If
you would like to use or CIM®
monolithic supports for any particular application,
which is not listed here, please do not hesitate to
contact us for further information at: sales@biaseparations.com
PURIFICATION OF PROTEINS AND PEPTIDES
| A001: |
Separation
of Three Standard Proteins and Study of Batch-to-Batch
Reproducibility on CIM®
DEAE Disks |
| A003: |
Separation
of Lignin Peroxidase Isoenzymes on a CIM®
QA Disk
For additional information please
see the following: Poster
|
| A004: |
Conjoint
Liquid Chromatography (CLC) Separation of Proteins
from Mouse Ascites and Isolation of Monoclonal Antibody
IgG in One Step Obtained by a Combination of CIM®
QA and CIM® Protein
A Disks
For additional information please
see the following: Poster
|
| A005: |
In-process
Analysis During the Purification of Factor VIII
from Human Plasma Obtained with a CIM®
QA Disk |
| A006: |
Step
Gradient Separation of Synthetic Peptide Mixture
on CIM® SO3 Disk |
| A007: |
Isocratic
Fractionation of Synthetic Peptide on CIM®
SO3 Disk |
| A008: |
Step
Gradient Separation of Protein Mixture on CIM®
C4 Disk |
| A009: |
Quantitative
Determination of Human IgG by High-Performance Immunoaffinity
Chromatography Using CIM®
Protein A Disk |
| A010: |
Isolation
of Anti-Bradikinin IgGs from Crude Serum of Immunized
Rabbit by High-Performance Immunoaffinity Chromatography
Using CIM® Bradikinin
Disk |
| A012: |
In
a Minute Quality Control of Human Serum Albumin
(HSA) Purity with CIM®
Disk Monolithic Columns |
| A013: |
Purification
of GTPgammaS Binding Proteins from Membranes of
Porcine Brain
For additional information please
see the following: Poster
|
| A014: |
Purification
of Lipase UL (Rhizopus sp.Q119) using a CIM® DEAE
disk monolithic column |
| A015: |
Comparison
of the Separation of BSA on Competitor Column and
CIM® QA Disk Monolithic Column |
| A016: |
Separation
of Casein Isoforms on CIM® QA Disk Monolithic Column |
| A018: |
Separation
of Synthetic Peptides on a CIM® QA Disk Monolithic
Column |
| A019: |
Separation of IgG, HSA and IgM standard on CIM® EDA monolithic column |
| P001: |
Two
Step Purification of a 66 kDa-Endoxylanase and a
Broad Specificity Endoglucanase/Xylanase from Prevotella
bryantii B14 on a CIM®
DEAE-8 Tube
For additional information please
see the following: Poster
|
| P002: |
Separation
of Conalbumin and Soybean Trypsin Inhibitor on a
CIM® DEAE-8 Tube Monolithic
Column: Influence of the Flow Rate on the Resolution
under the Conditions of a Linear Gradient |
| P003: |
Capacity
Determination for Binding Human IgG to a CIM®
SO3-8 Tube Monolithic Column
For additional information please
see the following: Poster
|
| P004: |
Productivity
of Semi-Preparative Isolation of Immunoglobulins
G from Human Plasma on CIM®
Disk and CIM® Tube Protein
G Monolithic Columns Versus Particle Based Agarose
Supports
For
additional information please see the following:
Poster
|
| P006: |
Purification
of D-Lactate Dehydrogenase by CIM®
DEAE-8 Tube Monolithic Column
|
PURIFICATION
OF OLIGO- AND POLYNUCLEOTIDES
| A002: |
Separation
of 8, 10, 12, 14, 15 and 16mer Oligodeoxynucleotides
on a CIM® DEAE Disk
For
additional information please see the following:
Poster
|
| A011: |
High-Performance
Monolith Chromatography of Different Forms of Plasmid
DNA by CIM® DEAE Disk
Using Different Gradient Slopes |
| P005: |
Anion
Exchange Semi-Preparative Purification of a 16-mer
Oligodeoxynucleotide on a CIM®
DEAE Disk Monolithic Column
For additional information please
see the following: Poster
|
PURIFICATION OF OTHER BIOMOLECULES
| A017: |
Purification
of Proteolytic Enzyme Stem Bromelain on CIM® QA
Disk Monolithic Column |
A.
PODGORNIK, M. BARUT, J. JANCAR, A. STRANCAR, T. TENNIKOVA.
High-performance membrane chromatography of
small molecules. Anal. Chem., 1999,
71, 2986-2991.
M.
VODOPIVEC, A. PODGORNIK, M. BEROVIC, A. STRANCAR. Application
of Convective Interaction Media (CIM)®
Disk Monolithic Columns for Fast Separation and Monitoring
of Organic Acids. JCS, 2000, Vol.
38, No. 11, 489-495.
USE AS BIOSENSORS RESP. BIOCONVERSIONS
Poster:
Application of CIM Disks with Immobilised Glucose Oxidase
for On-Line Glucose Measurement (PDF:
54KB)
M.
VODOPIVEC, M. BEROVIC, J. JANCAR, A.PODGORNIK, A. STRANCAR.
Application of Convective Interaction Media
disks with immobilised glucose oxidase for on-line glucose
measurements. Analytica Chimica Acta.,
2000, 407, 105-110.
C.
KASPER, J. HAGEDORN, R. FREITAG, T. TENNIKOVA. High
Performance Flow Injection Analysis by means of membrane
chromatography approach. J. Biotechnol.,
1999, 66, 3-12.
G.
A. PLATONOVA, M. A. SURZHIK, T. B. TENNIKOVA, G. P.
VLASOV, A. L. TIMKOVSKII. New bioreactor based
on immobilized polynucleotide phosphorylase.
Russ. Bioorg. Chem. (English), 1999, 25,
No.3, 166-171.
D.
JOSIC, H. SCHWINN, A. STRANCAR, A. PODGORNIK, M. BARUT,
Y.-P. LIM, VODOPIVEC, Martina. Use of compact,
porous units with immobilized ligands with high molecular
masses in affinity chromatography and enzymatic conversion
of substrates with high and low molecular masses.
J. Chromatogr. A, 1998, 803, 61-71.
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