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Conjoint Liquid Chromatography (CLC) Separation of Proteins from Mouse Ascites and Isolation of Monoclonal Antibody IgG in One Step Obtained by a Combination of CIM® QA and CIM® Protein A Disks

AN004-Proteins, IgG_QA disk_rProt A_2008
AN004-Proteins, IgG_QA disk_rProt A_2008

A sample of mouse ascites was subjected to separation of proteins and isolation of IgG by means of CLC. Configuration of CLC was chosen to have CIM® QA Disk (Anion Exchange Chromatography) as first and CIM® Protein A Disk (Affinity Chromatography) as second column. The single step operation resulted in baseline separation of proteins and IgG. Elution of proteins was affected by linear salt gradient elution, while elution of IgG was effected by a stepwise pH shift.

CIM® DEAE Disk (Diethylamino) (Pores 1.3 um)Information
CIM® r-Protein G Disk (Recombinant Protein G) (Pores 1.3 um)Information

Relevant applications

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