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Fast Monitoring of Bacteriophage Production During Fermentation Using the CIMac™ DEAE Analytical Column

AN025-Bacteriophage_CIMac DEAE_2010_1
AN025-Bacteriophage_CIMac DEAE_2010_1AN025-Bacteriophage_CIMac DEAE_2010_2AN025-Bacteriophage_CIMac DEAE_2010_3

Bacteriophages, viruses that infect bacteria, are being used as antibacterial agents, in phage display screening, as gene therapy delivery systems, and for bacteria typing. To use phages in these applications, they must be free of all impurities. A purification and concentration process was recently developed using an ion exchange monolithic column [1]. One of the key challenges faced in phage purification is the monitoring of genomic DNA (gDNA) released to the growth medium which can interfere with the various applications of phages. CIMac™ DEAE Analytical Columns can be used to monitor the fermentation process, evaluate the amount of degraded gDNA to determine the optimal fermentation endpoint and then to efficiently purify the phage particles.

CIMac™ QA-0.1 Analytical Column (Quaternary amine) (Pores 1.3 um)Information
CIMac™ DEAE-0.1 Analytical Column (Diethylamino) (Pores 1.3 um)Information

Relevant applications

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