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Plasmid DNA purification using novel HIC (C4) short monolithic columns

pDNA isoform separation was performed on CIM® C4 1 mL tube in linear descending salt gradient mode. Crude bacterial lysate containing pDNA was incubated with 4 M ammonium sulfate for 90 minutes at room temperature. Prior loading on CIM® C4 1 mL tube the solution was filtered. Elution and isoform separation was achieved by descending ammonium sulfate gradient and resulted in an sc:oc ratio of 99:1.  


CIMmultus™ HiP² Plasmid Process Pack™ 1-1 (1x DEAE 311.5114-2, 1x C4 HLD 311.8136-2)Information